毛**是細(xì)胞中高活性的團(tuán)體。它來(lái)源于真皮的間葉細(xì)胞，位于毛囊的基部。毛**能控制頭發(fā)生長(zhǎng)周期，誘導(dǎo)表皮毛囊發(fā)育并生成毛發(fā)纖維。培養(yǎng)早期，毛**細(xì)胞能誘導(dǎo)毛發(fā)體外生長(zhǎng)，但進(jìn)一步培養(yǎng)，該特性消失。毛發(fā)生長(zhǎng)受到毛囊細(xì)胞與間葉上皮細(xì)胞間相互作用的調(diào)節(jié)。例如：毛**細(xì)胞分泌的β干擾素抑制體外培養(yǎng)中外根鞘細(xì)胞的生長(zhǎng)。毛**細(xì)胞的存在也受到信號(hào)轉(zhuǎn)導(dǎo)途徑的影響，激活的ERK和Akt促進(jìn)毛**細(xì)胞的存活。
人毛**細(xì)胞提取于人毛**組織，一代凍存。每管含有細(xì)胞數(shù)>5x10^5cells/ml，此細(xì)胞通過(guò)纖維粘連蛋白和CD105免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：間充質(zhì)干細(xì)胞培養(yǎng)基 (MSCM, Cat. #7501)
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貨號(hào)
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2400
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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HHDPC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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[1] Filsell, W., Little, J.C., Stones, A.J., Granger, S.P. and Bayley, S.A. (1994) Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype. Journal of Cell Science 107:1761-1772.
[2] Kim, C.D., Choe, Y., Shim, C., Kim, K. (2002) Interferon Beta secreted from human hair dermal papilla cells inhibits the growth of outer root sheath cells cultured in vitro. Biochem Biophys Res Commun. 290:1133-8.
[3] Han, J.H., Kwon, O.S., Chung, J.H., Cho, K.H., Eun, H.C., Kim, K.H. (2004) Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 34:91-8.
Question:
Hi, I saw the subculturing method and I understood about poly L lysine
coating for maintaining the cells. And I'm wondering if I have to do
coating with poly L lysine even in a 96 well plate for some kinds of
assays. Without the coating, is it going to be affected to the cells?
Answers:
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Posted by Karishma Shah on Thursday, October 20, 2016
Dear customer,
Yes, poly-l-lysine must be used to, especially for a 96-well plate.
Primary cells generally need a substrate to attach to and the TC coating
on the plate or flask is usually not sufficient. Sometimes attachment
on 96 –well plates can be challenging, so we would definitely recommend
using poly-l-lysine.
