成纖維細(xì)胞是來(lái)源于中胚層的一種間充質(zhì)細(xì)胞。它被廣泛應(yīng)用于細(xì)胞和分子生物學(xué)的研究。這主要是因?yàn)樗且环N較容易培養(yǎng)的，耐受性較好的細(xì)胞，可應(yīng)用于基因轉(zhuǎn)染、顯微注射等多種操作?，F(xiàn)已證明不同部位的成纖維細(xì)胞有著本質(zhì)的不同，組織里的成纖維細(xì)胞處于一種動(dòng)態(tài)的平衡之中，這種平衡影響著健康，以及已**軟組織的結(jié)構(gòu)完整性。成纖維細(xì)胞分泌一種非剛性細(xì)胞外基質(zhì)，其中含有I型和III型膠原質(zhì)。此外真皮纖維細(xì)胞在炎癥刺激下還能分泌大量透明質(zhì)酸。在創(chuàng)傷**過(guò)程中，真皮成纖維細(xì)胞從遷移、再生表型轉(zhuǎn)變?yōu)榭墒湛s、基質(zhì)聚集表型。
人真皮成纖維細(xì)胞(**)提取于人皮膚組織，原代凍存。每管含有細(xì)胞數(shù)>5x10^5cells/ml，此細(xì)胞通過(guò)Fibronectin免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：成纖維細(xì)胞培養(yǎng)基 (FM, Cat. #2301)
|
貨號(hào)
|
2320
|
|
產(chǎn)地
|
美國(guó)
|
|
縮寫
|
HDF-a
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) Rouabhia M, Park H, Meng S, Derbali H, Zhang Z. (2013) "Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation." PLoS One. 8: e71660.
2.) Ploeger DT, Hosper NA, Schipper M, Koerts JA, de Rond S, Bank RA. (2013) "Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts." Cell Commun Signal. 11: 29. doi: 10.1186/1478-811X-11-29.
3.) Huang SM, Cheung CW, Chang CS, Tang CH, Liu JF, Lin YH, Chen JH, Ko SH, Wong KL, Lu DY. (2011) "Phloroglucinol derivative MCPP induces cell apoptosis in human colon cancer." J Cell Biochem. 112: 643-52.
4.) Kang TH, Guibinga GH, Jinnah HA, Friedmann T. (2011) "HPRT deficiency coordinately dysregulates canonical Wnt and presenilin-1 signaling: a neuro-developmental regulatory role for a housekeeping gene " PLoS One. 6: e16572.
5.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) " Multi layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. " Biomaterials. 30: 1587-95.
6.) Hao S, Baltimore D. (2009) "The stability of mRNA influences the temporal order of the induction of genes encoding inflammatory molecules." Nat Immunol. 10: 281-8.
7.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) "Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication." Biomaterials. 30: 1587-95.
8.) Montes CL, Chapoval AI, Nelson J, Orhue V, Zhang X, Schulze DH, Strome SE, Gastman BR. (2008) "Tumor-induced senescent T cells with suppressor function: a potential form of tumor immune evasion." Cancer Res. 68: 870-9.
There are no product questions yet.
