人黑色素細(xì)胞是神經(jīng)嵴衍生細(xì)胞，位于人的某些器官，如皮膚、眼睛、內(nèi)耳和軟腦膜。黑色素細(xì)胞不能向這些器官的移動，可以解釋先天性皮膚白斑(斑駁病)跟虹膜異色(不同顏色并置)之間的關(guān)系以及Waardenburg綜合征的先天性耳聾。皮膚黑色素細(xì)胞合成并轉(zhuǎn)運(yùn)黑色素到周圍的角質(zhì)細(xì)胞，使皮膚有色素沉積以保護(hù)皮膚對抗陽光輻射。隨著新的細(xì)胞培養(yǎng)技術(shù)和改良的培養(yǎng)方法，在成功培養(yǎng)這類細(xì)胞在純種群時，發(fā)現(xiàn)了新型的黑色素細(xì)胞基因——msgl，它能編碼一種與色素沉著相關(guān)的核蛋白。
人表皮黑色素細(xì)胞(深色素)提取于人表皮組織，一代凍存。每管含有細(xì)胞數(shù)>5x10^5cells/ml，此細(xì)胞通過Fibronectin和NGF-receptor(p75)免疫熒光染色驗(yàn)證，經(jīng)測試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：黑色素細(xì)胞培養(yǎng)基 (MelM, Cat. #2201)
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貨號
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2230
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產(chǎn)地
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美國
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縮寫
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HEM-a
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲存
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液氮
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運(yùn)輸
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干冰
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1.) Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, S?f S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, and Lesinski1 GB. (2013) "PRMT5 Is Upregulated in Malignant and Metastatic Melanoma and Regulates Expression of MITF and p27Kip1." PLoS One. 8: e74710.
2.) Looi CY, Moharram B, Paydar M, Wong YL, Leong KH, Mohamad K, Arya A, Wong WF, Mustafa MR. (2013) "Induction of apoptosis in melanoma A375 cells by a chloroform fraction of Centratherum anthelminticum (L.) seeds involves NF-kappaB, p53 and Bcl-2-controlled mitochondrial signaling pathways." BMC Complement Altern Med. 13: 166.
3.) Rotte A, Bhandaru M, Cheng Y, Sjoestroem C, Martinka M, and Li G. (2013) "Decreased Expression of Nuclear p300 Is Associated with Disease Progression and Worse Prognosis of Melanoma Patients." PLoS One. 8: e75405.
Question:
I purchased a HEM-a cell in February 2014 from ScienCell. Can you supply DNA (STR) profiling of this primary cell?
Thanks.
Answers:
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Posted by Manoj Sharma on Thursday, January 7, 2016
Hello,
We do not have STR profiling. thanks for your interest
SCIENCELL
