人黑色素細(xì)胞是神經(jīng)嵴衍生細(xì)胞，位于人的某些器官，如皮膚、眼睛、內(nèi)耳和軟腦膜。黑色素細(xì)胞不能向這些器官的移動(dòng)，可以解釋先天性皮膚白斑（斑駁病）跟虹膜異色(不同顏色并置)之間的關(guān)系以及Waardenburg綜合征的先天性耳聾。皮膚黑色素細(xì)胞合成并轉(zhuǎn)運(yùn)黑色素到周圍的角質(zhì)細(xì)胞，使皮膚有色素沉積以保護(hù)皮膚對(duì)抗陽光輻射。隨著新的細(xì)胞培養(yǎng)技術(shù)和改良的培養(yǎng)方法，在成功培養(yǎng)這類細(xì)胞在純種群時(shí)，發(fā)現(xiàn)了新型的黑色素細(xì)胞基因——msgl，它能編碼一種與色素沉著相關(guān)的核蛋白。
人表皮黑色素細(xì)胞(淺色素)提取于人表皮組織,一代凍存。每管含有細(xì)胞數(shù)>5x10^5cells/ml，此細(xì)胞通過Fibronectin和NGF-receptor(p75)免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：黑色素細(xì)胞培養(yǎng)基 (MeIM, Cat. #2201)
|
貨號(hào)
|
2200
|
|
產(chǎn)地
|
美國(guó)
|
|
縮寫
|
HEM-l
|
|
規(guī)格
|
5 x 10^5 /1ml
|
|
用途
|
科研
|
|
儲(chǔ)存
|
液氮
|
|
運(yùn)輸
|
干冰
|
1.) Adini I, Ghosh K, Adini A, Chi ZL, Yoshimura T, Benny O, Connor KM, Rogers MS, Bazinet L, Birsner AE, Bielenberg DR, D'Amato RJ."Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment." 2014 Jan;124(1):425-36. doi: 10.1172/JCI69404. Epub 2013 Dec 20.
2.) Yang J, Bill MA, Young GS, La Perle K, Landesman Y, Shacham S, Kauffman M, Senapedis W, Kashyap T, Saint-Martin JR, Kendra K, Lesinski GB. (2014) 'Novel Small Molecule XPO1/CRM1 Inhibitors Induce Nuclear Accumulation of TP53, Phosphorylated MAPK and Apoptosis in Human Melanoma Cells.'
3.) Yang J, Bill MA, Young GS, La Perle K, Landesman Y, Shacham S, Kauffman M, Senapedis W, Kashyap T, Saint-Martin JR, Kendra K, Lesinski GB. (2014) "Novel Small Molecule XPO1/CRM1 Inhibitors Induce Nuclear Accumulation of TP53, Phosphorylated MAPK and Apoptosis in Human Melanoma Cells." PloS one. 9: e102983.
4.) Li JL, Mazar J, Zhong C, Faulkner GJ, Govindarajan SS, Zhang Z,Dinger ME, Meredith G, Adams C, Zhang S, Mattick JS, Ray A, Perera RJ (2013) 'Genome-wide methylated CpG island profiles of melanoma cells reveal a melanoma coregulation network.'
5.) Li JL, Mazar J, Zhong C, Faulkner GJ, Govindarajan SS, Zhang Z,Dinger ME, Meredith G, Adams C, Zhang S, Mattick JS, Ray A, Perera RJ (2013) "Genome-wide methylated CpG island profiles of melanoma cells reveal a melanoma coregulation network." . 3.
6.) Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, Ray A, Perera RJ. (2010) "The regulation of miRNA-211 expression and its role in melanoma cell invasiveness." PLoS One. 5: e13779.
7.) Savaraj N, You M, Wu C, Wangpaichitr M, Kuo MT, Feun LG. (2010) "Arginine deprivation, autophagy, apoptosis (AAA) for the treatment of melanoma." Curr Mol Med. 10: 405-12.
8.) Doudican N, Rodriguez A, Osman I, Orlow SJ. (2008) "Mebendazole induces apoptosis via Bcl-2 inactivation in chemoresistant melanoma cells." Mol Cancer Res. 6: 1308-15.
9.) Palmer MB, Majumder P, Green MR, Wade PA, Boss JM. (2007) "A 3' Enhancer Controls Snail Expression in Melanoma Cells." Cancer Res. 67: 6113-20.
10.) May KM, Vogt A, Bachas LG, Anderson KW. (2005) "Vascular endothelial growth factor as a biomarker for the early detection of cancer using a whole cell-based biosensor." Anal Bioanal Chem. 382: 1010-6.
There are no product questions yet.
