皮膚上皮層是一層保護(hù)機(jī)體免受外界環(huán)境損傷的重要屏障。該層主要為角質(zhì)細(xì)胞類型，包含了大約85%活的表皮細(xì)胞。角質(zhì)層是復(fù)扁平上皮細(xì)胞。角質(zhì)細(xì)胞因含有大量的角蛋白而得名，在表皮基底層,角質(zhì)細(xì)胞分化、增殖，隨著角質(zhì)細(xì)胞產(chǎn)生角蛋白并上移至表皮表面后，發(fā)生程序性死亡。角蛋白細(xì)胞增殖、分化及凋亡是復(fù)雜且嚴(yán)格控制的過程。角蛋白細(xì)胞可產(chǎn)生很多細(xì)胞因子和生長因子，包括IL-1,IL-3,IL-6,IL-8,集落刺激因子,TNF-alpha,TGF-alpha and beta,成纖維細(xì)胞生長因子, 雙調(diào)蛋白和PDGF。除了保護(hù)功能之外，角蛋白細(xì)胞在組織穩(wěn)態(tài)、傷口恢復(fù)、癌癥以及皮膚基因治療上有著重要的作用。人角蛋白細(xì)胞能表達(dá)粘附分子和細(xì)胞因子,這說明了它能參與皮膚免疫和動(dòng)態(tài)平衡。
人表皮角質(zhì)細(xì)胞(新生)提取于新生兒的表皮組織，原代凍存。每管含有細(xì)胞數(shù)>5x10^5cells/ml，此細(xì)胞通過Cytokeratine-8,-18 and-19免疫熒光染色驗(yàn)證，經(jīng)測試不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌。細(xì)胞可以達(dá)到15倍增。
推薦培養(yǎng)基：角質(zhì)細(xì)胞培養(yǎng)基 (KM, Cat. #2101)
Question:
How many times can we Passage these Cells??
Answers:
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Posted by Manoj Sharma on Monday, August 17, 2015
Cat #2120, as stated in the product sheet, are guaranteed to further
expand for 15 population doublings under the conditions provided by
ScienCell. Please see our Frequently Asked Questions section under
Technical Resources for the difference between population doubling and
passage number.
