小膠質(zhì)細(xì)胞是**神經(jīng)系統(tǒng)中的膠質(zhì)細(xì)胞類(lèi)型之一，是神經(jīng)膠質(zhì)細(xì)胞網(wǎng)絡(luò)的重要組成部分。小膠質(zhì)細(xì)胞出現(xiàn)在大腦由發(fā)育早期向成熟階段轉(zhuǎn)化的過(guò)程中，被認(rèn)為是大腦免疫監(jiān)視的主要細(xì)胞。小膠質(zhì)細(xì)胞也表達(dá)?II類(lèi)主要組織相容性復(fù)合體（MHC?II），向CD4陽(yáng)性T細(xì)胞呈遞抗原，并能進(jìn)行Fc段介導(dǎo)的胞吞作用，并且與造血細(xì)胞和組織巨噬細(xì)胞分享多種抗原。在大腦發(fā)育或**神經(jīng)系統(tǒng)受傷發(fā)生程序性細(xì)胞死亡后，小膠質(zhì)細(xì)胞能被激活。做為大腦的巨噬細(xì)胞清除組織碎片，受損細(xì)胞或微生物。另外，有大量的證據(jù)表明，小膠質(zhì)細(xì)胞參與多種的生理和病理過(guò)程，它們通過(guò)與神經(jīng)元和其它膠質(zhì)細(xì)胞共同作用，產(chǎn)生諸如生長(zhǎng)因子、細(xì)胞因子等活性物質(zhì)來(lái)完成以上功能。
ScienCell實(shí)驗(yàn)室的HM提取自大腦皮層，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)1×106/ml。細(xì)胞經(jīng)OX-42（CD11b/c）和F4/80免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1,?HBV,?HCV,?支原體,?細(xì)菌,?酵母菌和真菌。如采用ScienCell?實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞生長(zhǎng)良好。HM不推薦增值培養(yǎng)。
推薦培養(yǎng)基：小膠質(zhì)細(xì)胞培養(yǎng)基(MM, Cat. #1901)
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貨號(hào)
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1900
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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HM
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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4.) Campbell GR, Watkins JD, Singh KK, Loret EP, Spector SA. (2007) "Human Immunodeficiency Virus Type 1 Subtype C Tat Fails To Induce Intracellular Calcium Flux and Induces Reduced Tumor Necrosis Factor Production from Monocytes." J Virol. 81: 5919-28.
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1、Question:
Dear whom it may concern, I’m wondering whether your microglia express chemokine receptors particularly, CD36, TLR3, TLR4, TLR7. Any finding by you or your customers are valuable for me to use your cells as the model. Thanks, H.Cho
Answers:
We do not test for the receptors listed. Thank you for your interest in ScienCell products
2、Question:
Hello, Do these human microglia immortalized cells express CD80/86 as normal microglia do when stimulated? Also, are there any publications that utilized this cell line for flow cytometryic analysis? Thanks
Answers:
Human microglia provided by ScienCell are primary cells, not immortalized cell lines. Thank you for your interest
