雪旺細(xì)胞是神經(jīng)嵴的衍生物，形成周圍神經(jīng)髓磷脂軸突的髓鞘。它們分別環(huán)繞在周圍神經(jīng)軸突的軸上，沿著軸突節(jié)形成一層髓磷脂髓鞘。雪旺細(xì)胞對(duì)周圍神經(jīng)的發(fā)育，功能和再生起著重要作用。當(dāng)軸突快死亡時(shí)，雪旺細(xì)胞環(huán)繞其周圍幫助消化軸突。留下連續(xù)的雪旺細(xì)胞形成一個(gè)空的管道，新的軸突在管道的末端開始生長。在周圍神經(jīng)中雪旺細(xì)胞的數(shù)量是受到嚴(yán)格調(diào)控的。體外增殖受到諸如PDGF，F(xiàn)GF，神經(jīng)元和其它多肽類生長因子的刺激。雪旺細(xì)胞提供相對(duì)簡單，明確，易得的哺乳動(dòng)物模型以用來研究一系列發(fā)育問題。了解血旺細(xì)胞的生物學(xué)特征具有重要的**意義，不僅對(duì)于研究神經(jīng)病變產(chǎn)生的環(huán)境和神經(jīng)的再生，而且細(xì)胞或他們的前體可能特別適合用作**神經(jīng)系統(tǒng)修復(fù)的植入物。
ScienceCell實(shí)驗(yàn)室的HSC提取自人脊髓神經(jīng)，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過5×105/ml。細(xì)胞經(jīng)S-100,?GFAP?和?CD90免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HSC?HIV-1,?HBV,?HCV,?支原體,?細(xì)菌,?酵母菌和真菌。如采用ScienCell?實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞10次倍增。? ?
推薦培養(yǎng)基：雪旺細(xì)胞培養(yǎng)基(SCM, Cat. #1701)
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貨號(hào)
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1700
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產(chǎn)地
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美國
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縮寫
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HSC
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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6.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) '12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy.'
7.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) "12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy." J diabetes mellitus. 3.
8.) Peng J, Wang Y, Zhang L, Zhao B, Zhao Z, Chen J, Guo Q, Liu S, Sui X, Xu W, Lu S. (2011) "Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro." Brain Res Bull. 84: 235-43.
9.) Ahmad Z, Brown CM, Patel AK, Ryan AF, Ongkeko R, Doherty JK. (2010) "Merlin knockdown in human Schwann cells: clues to vestibular schwannoma tumorigenesis." Otol Neurotol. 31: 460-66.
10.) Arima Y, Hayashi H, Kamata K, Goto TM, Sasaki M, Kuramochi A, Saya H. (2010) "Decreased expression of neurofibromin contributes to epithelial-mesenchymal transition in neurofibromatosis type 1." Exp Dermatol. 19: e136-41.
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12.) Stavniichuk R, Drel VR, Shevalye H, Vareniuk I, Stevens MJ, Nadler JL, Obrosova IG. (2010) "Role of 12/15-lipoxygenase in nitrosative stress and peripheral prediabetic and diabetic neuropathies." Free Radic Biol Med. 49: 1036-45.
13.) Suenaga T, Satoh T, Somboonthum P, Kawaguchi Y, Mori Y, Arase H. (2010) "Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses." Proc Natl Acad Sci USA. 107: 866-71.
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16.) Askwith T, Zeng W, Eggo MC, Stevens MJ. (2009) "Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy." Am J Physiol Endocrinol Metab. 297: E620-28.
17.) Bodempudi V, Yamoutpoor F, Pan W, Dudek AZ, Esfandyari T, Piedra M, Babovick-Vuksanovic D, Woo RA, Mautner VF, Kluwe L, Clapp DW, De Vries GH, Thomas SL, Kurtz A, Parada LF, Farassati F. (2009) "Ral overactivation in malignant peripheral nerve sheath tumors." Mol Cell Biol. 29: 3964-74.
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20.) Obrosova IG, Drel VR, Pacher P, Ilnytska O, Wang ZQ, Stevens MJ, Yorek MA. (2005) "Oxidative-nitrosative stress and poly(ADP-ribose) polymerase (PARP) activation in experimental diabetic neuropathy - The relation is revisited." Diabetes. 54: 3435-41.
1、Question:
What is the best way you suggest to cryopreserve primary human Schwann cells?
Answers:
We do not recommend that customers re-freeze our cells since primary cells are fragile and they may be damaged during re-freeze and re-thaw process.
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2、Question:
In the product description, these cells were analyzed by immunofluorescence using antibodies against S100, GFAP, and CD90. Would you be able to provide manufacturer/catalog# for those antibodies? Your protocols for cell preparation and immunofluorescence using those antibodies would be kindly appreciated as well? Thank you
Answers:
Posted by Manoj Sharma on Thursday, January 14, 2016 Dear Amish, Here are the antibodies we use for staining #1700 Human Schwann Cells: （1）S-100 beta: Sigma Cat. No. S2532 1:500 dilution （2）GFAP: Sigma Cat. No. G3893 1:1000 dilution And following is the protocol that we use for staining the cells. Immunofluorescence Staining Protocol a. Fixing the slide i. Wash each well with 500 μl PBS making sure to add along the side of the well wall. ii. Add 500 μl 4% PFA (paraformaldehyde) to each well and place at room temperature for 5 minutes. b. Blocking Solution i. Wash each well with 500 μl PBS. ii. The blocking solution consists of 5% NGS (Normal Goat Serum) and 0.1% Triton-X-100 in PBS. iii. The blocking solution can be made in bulk in a 15mL conical tube and then placed in the wells. iv. For every 1 ml of PBS add 50 μl of NGS and 10 μl of 10% Triton-X 100. Add 250 μl per well and incubate at room temperature for 1 hour. c. Primary Antibody i. Prepare the solution for the primary antibody. This consists of 1% NGS and 1% Triton-X 100 in PBS. For every 1 ml of PBS add 10 μl of NGS and 10 μl of Triton-X 100. This can be made in bulk in a conical tube. ii. Dilute each primary antibody needed with the necessary dilution factor (depending on the antibody used) and add 250 μl to the appropriate wells. Place the slide in 4°C overnight. Dilutions can be made in 1.5mL microcentrifuge tubes. iii. If the slide needs be stained on the same day, it can be placed in the 37°C incubator for 2 hours instead of 4°C overnight. iv. Make sure to note on the Immunocytochemistry form the specific antibody placed in the well. d. Secondary Antibody i. Wash the slide 3 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. ii. Dilute each secondary antibody (usually 1:1000 to 1:2000, depending on the intensity) needed with PBS and add 250 μl to the appropriate wells. Incubate at 37°C in the incubator for 45 minutes. iii. Wash the slide 5 times with 500 μl PBS leaving the PBS in the wells 5-7 minutes per rinse. iv. The secondary antibody is specific to the primary antibody such as IgG Rabbit or IgG Mouse. The animal it derives from must be the same as the primary antibody. e. Preparing the slide for microscopic examination i. Drop about 5-8 μl of mounting medium (DAPI) to a slide, remove cover slips from the well using tweezers and place them over the slide on the mounting medium gently. ii. Make sure the DAPI covers the entire area of the cover slips. f. Microscopic Examination Examine the slides after 20-30 minutes under fluorescence microscope. Thank you for your interest in ScienCell Products
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3、Question:
Has anyone used these cells to look at myelin production? I work with a virus that may disrupt myelination and I was interested in looking at this in primary tissue culture cells. Thanks! Lee
Answers:
We do not test for myelin production. thank you for your interest.
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