少突膠質(zhì)前體細(xì)胞是Raff、Miller 和Noble于1993年**發(fā)現(xiàn)[1]，并已進(jìn)行了深入的研究。在文獻(xiàn)中，前體細(xì)胞指少突細(xì)胞型星形膠質(zhì)細(xì)胞祖細(xì)胞或少突膠質(zhì)細(xì)胞前體細(xì)胞。發(fā)育中和成熟的**神經(jīng)系統(tǒng)都含有少突膠質(zhì)前體細(xì)胞[2, 3]。少突膠質(zhì)細(xì)胞，**神經(jīng)系統(tǒng)中的髓磷脂組成細(xì)胞，來源于少突前體細(xì)胞。在培養(yǎng)中，少突前體細(xì)胞在基本的成纖維細(xì)胞生長因子存在的情況下可產(chǎn)生于神經(jīng)祖細(xì)胞或神經(jīng)干細(xì)胞，OPC在血小板衍生的生長因子或星形膠質(zhì)細(xì)胞產(chǎn)生的生長因子[4]存在的情況下才能增殖，并分化成為成熟的少突膠質(zhì)細(xì)胞。正因如此，它們?yōu)檠芯堪l(fā)育轉(zhuǎn)型提供了特殊的細(xì)胞種群。
ScienCell實驗室的HOPC分離自人腦，原代凍存，冰凍運輸。每管細(xì)胞密度*過1×106/ml。細(xì)胞經(jīng) A2B5和O4免疫熒光鑒定。本細(xì)胞經(jīng)檢測不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養(yǎng)基，可保證此細(xì)胞生長良好，此細(xì)胞不推薦增殖培養(yǎng)。
推薦培養(yǎng)基：少突膠質(zhì)前體細(xì)胞培養(yǎng)基 (OPCM, Cat. #1601)
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貨號
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1600
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產(chǎn)地
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美國
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縮寫
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HOPC
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規(guī)格
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1 x 10^6 /1ml
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用途
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科研
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儲存
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液氮
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運輸
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干冰
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1.) Hoelzinger DB, Nakada M, Demuth T, Rosensteel T, Reavie LB, Berens ME. (2008) "Autotaxin: a secreted autocrine/paracrine factor that promotes glioma invasion." J Neurooncol. 86: 297-309.
2.) Qiu J, Ai L, Ramachandran C, Yao B, Gopalakrishnan S, Fields CR, Delmas AL, Dyer LM, Melnick SJ, Yachnis AT, Schwartz PH, Fine HA, Brown KD, Robertson KD. (2008) "Invasion suppressor cystatin E/M (CST6): high-level cell type-specific expression in normal brain and epigenetic silencing in gliomas." Lab Invest. 88: 910-25.
3.) Wollmann G, Robek MD, van den Pol AN. (2007) "Variable deficiencies in the interferon response enhance susceptibility to vesicular stomatitis virus oncolytic actions in glioblastoma cells but not in normal human glial cells." J Virol. 81: 1479-91.
4.) Kong H, Baerbig Q, Duncan L, Shepel N, Mayne M. (2003) "Human Herpesvirus Type 6 Indirectly Enhances Oligodendrocyte Cell Death." Journal of NeuroVirology 9: 539-50.
Question:
What is the size range of these cells?
Answers:
Each vial contains >1 x 10^6 cells in 1 ml volume.
