周細(xì)胞是具伸縮性的平滑肌細(xì)胞樣的細(xì)胞，它們覆蓋在微脈管的基底面。周細(xì)胞是小靜脈的主要成分，并普遍存在于毛細(xì)血管中。在不同的組織和器官中，周細(xì)胞的數(shù)量和功能均呈現(xiàn)很大的差異。腦血管周細(xì)胞的主要功能包括參與**神經(jīng)系統(tǒng)微血管收縮性，內(nèi)皮細(xì)胞活性及巨噬細(xì)胞活性的調(diào)節(jié)。也有證據(jù)表明周細(xì)胞與血腦屏障的物質(zhì)轉(zhuǎn)運(yùn)及血管滲透性的調(diào)節(jié)有關(guān)。病理學(xué)研究顯示，周細(xì)胞與高血壓，糖尿病視網(wǎng)膜病變，阿爾海默氏病，多發(fā)性硬化和**神經(jīng)系統(tǒng)腫瘤的形成有關(guān)。
ScienCell 實(shí)驗(yàn)室的HBVP分離自人大腦組織，原代凍存，冰凍運(yùn)輸。每管細(xì)胞密度*過(guò)5×105/ml。細(xì)胞經(jīng)α-smooth muscle actin免疫熒光鑒定。本細(xì)胞經(jīng)檢測(cè)不含HIV-1、HBV、HCV、支原體、細(xì)菌、酵母菌和真菌。如采用ScienCell 實(shí)驗(yàn)室特制的培養(yǎng)基，可保證此細(xì)胞達(dá)到15次或以上的倍增。
推薦培養(yǎng)基：周細(xì)胞培養(yǎng)基 (PM, Cat. #1201)
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貨號(hào)
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1200
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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HBVP
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規(guī)格
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5 x 10^5 /1ml
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用途
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科研
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儲(chǔ)存
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液氮
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運(yùn)輸
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干冰
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5.) Santos ARC, Dvoriantchikova G, Li Y, Mohammad G, El-Asrar AMA, Wen R, Ivanov D. (2014) "Cellular Mechanisms of High Mobility Group 1 (HMGB-1) Protein Action in the Diabetic Retinopathy." PLOS ONE. 9: e87574.
6.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
7.) Urich E, Patsch C, Aigner S, Graf M, Iacone R, Freskgrd P. (2013) "Multicellular self-assembled spheroidal model of the blood brain barrier." Sci Rep. 3: 1500.
8.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
9.) Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV. (2013) 'Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism.' PloS one.
10.) Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV. (2013) "Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism." PloS one. 8: e82900.
11.) Maier CL, Pober JS. (2011) "Human placental pericytes poorly stimulate and actively regulate allogeneic CD4 T cell responses." Arterioscler Thromb Vasc Biol. 31: 183-89.
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13.) Stratman AN, Schwindt AE, Malotte KM, Davis GE. (2010) "Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization." Blood. 116: 4720-30.
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16.) El-Bizri N, Guignabert C, Wang L, Cheng A, Stankunas K, Chang CP, Mishina Y, Rabinovitch M. (2008) "SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis." Development. 135: 2981-91.
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19.) Bagley RG, Weber W, Rouleau C, Teicher BA. (2005) "Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy." Cancer Res. 65: 9741-50.
Question:
Do you have an STR profile available for Human Brain Vascular Pericytes #1200?
Answers:
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Posted by Manoj Sharma on Thursday, April 21, 2016
Hi Daniel,
We apologize for the inconvenience, but the STR profile for Catalog
#1200 is not currently available. Please email your request to TechSupport@sciencellonline.com and we will respond as soon as possible.”
Best,
Sciencell Team
Question:
Are they GFAP positive?
Answers:
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Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi duan,
Human Brain Vascular Pericytes are NOT GFAP positive.
Thank you
Question:
How are these cells isolated? Are they PDGFRb+? Do you know if they express other pericyte markers like NG2?
Answers:
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Posted by Karishma Shah on Tuesday, March 29, 2016
Dear customer,
Isolation method is proprietary. #1200 cells express both PDGF beta receptor and NG2.
Question:
Do you know the ApoE genotype information of these human pericytes? ApoE2, ApoE3 or ApoE4. Can request a specific one?
Answers:
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Posted by Manoj Sharma on Thursday, January 7, 2016
Hi,
We do not test these. thanks for your interest, please email at techsupport@sciencellonline,.com for special services.
SCIENCELL TEAM
Question:
The spec sheet says you can passage these cells 15 times, but I don't
see it listed anywhere that they are immortalized. Please clarify.
Answers:
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Posted by Manoj Sharma on Thursday, January 7, 2016
Hi Susan,
The cells can proliferate for 15 population doublings, not 15
passages. Cells may reach 15 population doublings within 4-5 passages.
thanks for your interest
SCIENCELL
