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Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

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Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

品牌：

貨號：EKU02559

規(guī)格：96T

貨期：

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Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

商品詳情參考文獻(xiàn) 相關(guān)資料

Species Reactivity	Rattus norvegicus (Rat)
Format	96 well plate
Detection Range	0.78-50 ng/mL
Sensitivity	0.27 ng/mL
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Assay Time	3 hours
Gene Name	Arachidonate-5-Lipoxygenase
Gene Aliases	LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Arachidonate-5-Lipoxygenase (ALOX5). No significant cross-reactivity or interference between Arachidonate-5-Lipoxygenase (ALOX5) and analogues was observed.
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-5-Lipoxygenase (ALOX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-5-Lipoxygenase (ALOX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-5-Lipoxygenase (ALOX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-5-Lipoxygenase (ALOX5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science;