|
Species Reactivity |
Rattus norvegicus (Rat) |
|
Format |
96 well plate |
|
Detection Range |
3.12-200 ng/mL |
|
Sensitivity |
5.1 ng/mL |
|
Sample Type |
Serum, plasma, tissue homogenates and other biological fluids |
|
Assay Time |
3 hours |
|
UniProt |
P10719 |
|
Gene ID |
171374 |
|
Gene Name |
ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide |
|
Gene Aliases |
ATPMB; ATPSB |
|
Application |
ELISA |
|
Assay Type |
Sandwich |
|
Detection Method |
Colorimetric |
|
Shipping Condition |
Blue ice |
|
Storage Instructions |
-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
|
Precaution Of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
|
Expiry Date |
8 months |
|
Specificity |
This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). No significant cross-reactivity or interference between ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) and analogues was observed. |
|
Test Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
|
Assay Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision betw |
|
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
|
Restrictions |
For research use only. Not for diagnostic procedures. |
|
Quality Systems |
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
|
Research Area |
Signal transduction; |
