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Human Lecithin Cholesterol Acyltransferase (LCAT) CLIA Kit

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Human Lecithin Cholesterol Acyltransferase (LCAT) CLIA Kit

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貨號：EKU09980

規(guī)格：96T

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Human Lecithin Cholesterol Acyltransferase (LCAT) CLIA Kit

商品詳情參考文獻相關資料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	2.34-150 ng/mL
Sensitivity	0.1 ng/mL
Sample Type	Serum, plasma and other biological fluids
Assay Time	2 hours, 40min
UniProt	P04180
Gene ID	3931
Gene Name	Lecithin Cholesterol Acyltransferase
Gene Aliases	phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
Application	CLIA
Assay Type	Sandwich
Detection Method	Chemiluminescent
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.
Test Principle	The microplate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lecithin Cholesterol Acyltransferase (LCAT) level in the sample or standard.;
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Enzyme & Kinase;