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Human Chorionic Gonadotropin (hCG) ELISA Kit

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Human Chorionic Gonadotropin (hCG) ELISA Kit

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貨號：EKD01005

規(guī)格：96T

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Human Chorionic Gonadotropin (hCG) ELISA Kit

商品詳情參考文獻相關資料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	0-500 IU/L
Sensitivity	0.7 IU/L
Sample Type	Human Serum
Assay Time	2 hours
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Ambient shipping
Storage Instructions	4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	1 year
Test Principle	The principle of the following enzyme immunoassay test follows a typical one-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for hCG is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of hCG is conjugated to horse radish peroxidase (HRP). hCG from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of hCG in the sample. A set of standards is used to plot a standard curve from which the amount of hCG in patient samples and controls can be directly read. Please note that a two-step procedure is included and is to be used for assaying serum from pregnant women.
Assay Precision	The intra assay precision is between a CV% of 3.2-5.3 IU/L. The inter assay precision is between aCV% of 4.9-8.2 IU/L.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.