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Human Testosterone Saliva ELISA Kit

品牌：

貨號(hào)：EKD01032

規(guī)格：96T

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Human Testosterone Saliva ELISA Kit

商品詳情參考文獻(xiàn) 相關(guān)資料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	0-800 pg/ml
Sensitivity	1.0 pg/ml
Sample Type	Human Saliva
Assay Time	2 hours
Application	ELISA
Assay Type	Competitive
Detection Method	Colorimetric
Shipping Condition	Ambient shipping
Storage Instructions	4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	1 year
Test Principle	The principle of the following enzyme immunoassay test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and a biotin-labelled antigen (conjugate) for a limited number of antibody s on the microwell plate. In the second step, the streptavidin-horseradish peroxidase conjugate binds to any bound biotinylated testosterone.The washing and decanting procedures remove unbound materials. After the last washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.
Assay Precision	The intra assay precision is between a CV% of 3.4-7.1 pg/ml. The inter assay precision is between a CV% of 7.0-9.8 pg/ml.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.