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Mouse Substance P (SP) CLIA Kit

品牌：

貨號(hào)：EKU08616

規(guī)格：96T

貨期：

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Mouse Substance P (SP) CLIA Kit

商品詳情參考文獻(xiàn) 相關(guān)資料

Species Reactivity	Mus musculus (Mouse)
Format	96 well plate
Detection Range	11.72-3000 pg/mL
Sensitivity	3.8 pg/mL
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time	2 hours
Gene Name	Substance P
Application	CLIA
Assay Type	Competitive
Detection Method	Chemiluminescent
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Substance P (SP). No significant cross-reactivity or interference between Substance P (SP) and analogues was observed.
Test Principle	The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Substance P (SP). A competitive inhibition reaction is launched between biotin labeled Substance P (SP) and unlabeled Substance P (SP) (Standards or samples) with the pre-coated antibody specific to Substance P (SP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Substance P (SP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Substance P (SP) level in the sample or standard.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Substance P (SP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sub
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Endocrinology;Neuro science;Hormone metabolism;