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Rat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit

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Rat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit

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貨號(hào)：EKU09860

規(guī)格：96T

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Rat Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) CLIA Kit

商品詳情參考文獻(xiàn) 相關(guān)資料

Species Reactivity	Rattus norvegicus (Rat)
Format	96 well plate
Detection Range	234.38-15000 pg/mL
Sensitivity	7.3 pg/mL
Sample Type	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Time	2 hours, 40min
UniProt	Q00981
Gene ID	29545
Gene Name	Ubiquitin Carboxyl Terminal Hydrolase L1
Gene Aliases	PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
Application	CLIA
Assay Type	Sandwich
Detection Method	Chemiluminescent
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.
Test Principle	The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) level in the sample or standard.;
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples wit
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Enzyme & Kinase;