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Rat Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

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Rat Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

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貨號：EKU06832

規(guī)格：96T

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Rat Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

商品詳情參考文獻相關(guān)資料

Species Reactivity	Rattus norvegicus (Rat)
Format	96 well plate
Detection Range	0.156-10 ng/mL
Sensitivity	0.069 ng/mL
Sample Type	Tissue homogenates and other biological fluids.
Assay Time	3 hours
UniProt	P54001
Gene ID	64475
Gene Name	Prolyl-4-Hydroxylase Alpha Polypeptide I
Gene Aliases	Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). No significant cross-reactivity or interference between Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) and analogues was observed.
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples wit
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Enzyme & Kinase;