亚洲精品97久久中文字幕无码,欧美亚洲一区二区,丁香五月开心好婷婷网

您好，歡迎來到拜力生物網(wǎng)站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補體血清、兔補體、干擾素、小鼠測溫儀、進口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關于我們

所在位置：首頁 > 產(chǎn)品中心 > Biomatik > Biomatik產(chǎn)品

Human Polymerase DNA Directed Gamma 1 (POLg1) ELISA Kit

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Human Polymerase DNA Directed Gamma 1 (POLg1) ELISA Kit

品牌：

貨號：EKU06740

規(guī)格：96T

貨期：

應用：

咨詢客服

立即購買加入購物車

Human Polymerase DNA Directed Gamma 1 (POLg1) ELISA Kit

商品詳情參考文獻相關資料

Species Reactivity	Homo sapiens (Human)
Format	96 well plate
Detection Range	0.312-20 ng/mL
Sensitivity	0.114 ng/mL
Sample Type	Tissue homogenates, cell lysates and other biological fluids.
Assay Time	3 hours
UniProt	P54098
Gene ID	5428
Gene Name	Polymerase DNA Directed Gamma 1
Gene Aliases	PEO; POLG1; POLGA; SANDO; SCAE; Mitochondrial DNA polymerase catalytic subunit
Application	ELISA
Assay Type	Sandwich
Detection Method	Colorimetric
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Gamma 1 (POLg1). No significant cross-reactivity or interference between Polymerase DNA Directed Gamma 1 (POLg1) and analogues was observed.
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Gamma 1 (POLg1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Gamma 1 (POLg1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Gamma 1 (POLg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Gamma 1 (POLg1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymerase DNA Directed Gamma 1 (POLg1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mi
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Enzyme & Kinase;