|
Species Reactivity |
Homo sapiens (Human) |
|
Format |
96 well plate |
|
Detection Range |
78.12-5000 pg/mL |
|
Sensitivity |
30 pg/mL |
|
Sample Type |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
|
Assay Time |
3 hours |
|
UniProt |
P08590 |
|
Gene ID |
4634 |
|
Gene Name |
Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal |
|
Gene Aliases |
CMH8; MLC1V; VLC1; MLC1SB; Cardiac myosin light chain 1; Ventricular/slow twitch myosin alkali light chain |
|
Application |
ELISA |
|
Assay Type |
Sandwich |
|
Detection Method |
Colorimetric |
|
Shipping Condition |
Blue ice |
|
Storage Instructions |
-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
|
Precaution Of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
|
Expiry Date |
8 months |
|
Specificity |
This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). No significant cross-reactivity or interference between Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) and analogues was observed. |
|
Test Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
|
Assay Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) |
|
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
|
Restrictions |
For research use only. Not for diagnostic procedures. |
|
Quality Systems |
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
|
Research Area |
Metabolic pathway; |
