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Anti-CK-1α (Phospho-Tyr294) Antibody

品牌：Antibodies

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規(guī)格：50μl

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Anti-CK-1α (Phospho-Tyr294) Antibody

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Name:	Anti-CK-1α (Phospho-Tyr294) Antibody See all CK-1α primary antibodies
Description:	Rabbit polyclonal antibody to CK-1α (Phospho-Tyr294)
Specificity:	The antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.
Applications:	WB, IHC
Dilutions:	Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity:	Human
Immunogen:	Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α.
Host:	Rabbit
Clonality:	Polyclonal
Conjugate:	Unconjugated
Purification:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration:	1.0mg / ml
Formulation:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage:	Store at -20?C / 1 year

Function:	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).
Sequence Similarities:	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Cellular Location:	Cytoplasm. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Chromosome > Centromere > Kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton (PubMed:23902688).