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Anti-Caspase 9 (Phospho-Thr125) Antibody

品牌：Antibodies

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規(guī)格：50μl

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Anti-Caspase 9 (Phospho-Thr125) Antibody

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Name:	Anti-Caspase 9 (Phospho-Thr125) Antibody See all Caspase 9 primary antibodies
Description:	Rabbit polyclonal antibody to Caspase 9 (Phospho-Thr125)
Specificity:	The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).
Applications:	WB, IHC
Dilutions:	Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity:	Human
Immunogen:	Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.
Host:	Rabbit
Clonality:	Polyclonal
Conjugate:	Unconjugated
Purification:	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration:	1.0mg / ml
Formulation:	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage:	Store at -20?C / 1 year

Function:	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Tissue Specificity:	Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence Similarities:	Belongs to the peptidase C14A family.
Post-Translational Modification:	Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Synonyms:	APAF3 Antibody CASP9 Antibody Caspase 9 Antibody ICE LAP6 Antibody MCH6 Antibody
Information:	Target information shown above is from the UniProt Consortium.