Description
Viva SybrGreen Nucleic Acid Stain is one of a kind novel generation of fluorescent nucleic acid gel stain used to detect double-stranded DNA in agarose and polyacrylamide gels without requiring a destaining step. The detection limit using Viva SybrGreen Nucleic Acid Stain is as low as 60pg doublestranded DNA at 300nm transillumination and as little as 20pg double-stranded DNA can be detected at 254nm illumination. Single-stranded DNA and RNA can be detected at approximately 100pg to 300pg per well.
Viva SybrGreen Nucleic Acid Stain is designed to replace the highly toxic ethidium bromide (EtBr). The sensitivity is 25 times greater than ethidium bromide. Viva SybrGreen Nucleic Acid Stain is nontoxic and more sensitive than EtBr.
Features
Safer
The dye is noncytotoxic & nonmutagenic shown by Ames tests.
Higher sensitivity
5-10X more sensitive than EtBr under UV and 8-20X more sensitive than EtBr in visible light.
Extremely stable
Stable at room temperature for long-term storage. Stable to be microwaved or being heated. The working solution is stable at room temperature when kept in dark.
Wide application
Suitable to stain dsDNA, ssDNA and RNA. Suitable to use in agarose gel or polyacrylamide gel. Compatible with downstream applications, such as gel recovery & cloning.
Easy staining protocols
Easy precast gel staining, pre-staining and post-staining procedures.
Compatible with most imaging system
Gel can be viewed with standard UV transilluminator, visible light gel reader or other gel imaging system.
Storage Buffer
Shipped at ambient temperature Stored at 2-8℃ or -20℃
Recommended Equipment Required But Not Supplied:
-
ElitePro Single Channel Adjustable Volume Pipette, 1-10uL
(Hercuvan Cat No: TT-EP-10U)
-
MiniGEL Electrophoresis System
(Hercuvan Cat No: TT-HES-1-110V/TT-HES-1-220V)
-
SafeViewer Blue Light Transilluminator
(Hercuvan Cat No: TT-BLT-470)
Ordering Information
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Catalog No
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Description
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Pack Size
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SD0107
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Viva SybrGreen Nucleic Acid Stain (10000X in DMSO)
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1ml
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Download
Manual
Viva SybrGreen Nucleic Acid Stain (10000X in DMSO)
