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Mouse monoclonal antibody
BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
Purified human PAPP-A
IgG2b/k
The epitop of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.
Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
ELISA In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as detection antibody. BTE 004-09 can also be used as detection antibody in sandwich ELISA ?in combination with a polyclonal antibody towards PAPP-A in coat.?
WB BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.
IHC BTE 004-09 can be used in IHC. ?
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