都市激情综合,久久视频这里有精品63,果冻传媒天美传媒在线看

您好，歡迎來到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > BioPorto > BioPorto產(chǎn)品

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Anti-Gliadin peptide HYB 314-01

品牌：

貨號(hào)：HYB 314-01

規(guī)格：200 μg

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

Anti-Gliadin peptide

商品詳情參考文獻(xiàn) 相關(guān)資料

Cat. No.: HYB 314-01
Product name: Anti-Gliadin peptide
Description: Mouse monoclonal antibody
Specificity: HYB 314-01 reacts strongly with the deamidated peptide but cross-reacts about 5% with the non-deamidated peptide KLQPFPQPQLPYPQPQ.
Immunogen: Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ)?
Application: ELISA, WB
Species reactivity (positive): Wheat, Rye, Barley
Species reactivity (negative): Oat
Subclass: IgG2a/k
Clone number: 14D5
Epitope: The epitope is located in a central region of the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide (1).
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification: Protein-A purified
Form: Liquid
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8oC without exposure to light. No precautions necessary during handling.
Target: Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

Application 1: ELISA
HYB 314-01 reacts with both the deamidated peptide (KLQPFPQPELPYPQPQ) and non-deamidated petide (KLQPFPQPQLPYPQPQ) when these are coated directly in an immunoplate, but approx. 20-fold higher concentrations of antibody are needed to produce the same signal with the non-deamidated peptide. (2)
Application 2: WB
HYB 314-01 can be used in western blotting.
?

A western blot of Gliadin peptide (HYB 314-01) measured in rye (lane 1), barley (lane 2) and wheat (lane 3). The SDS gel was run under reducing conditions, and HYB 314-01 diluted 1:1000.

1. Hartwig Petersen N, Hansen PR, Houen G (2011) Fast and efficient characterization of an anti-gliadin monoclonal antibody epitope related to celiac disease using resin-bound peptides. J immunol Methods 365:174-182.: 21195715
2. Skovbjerg H, Koch C, Anthonsen D, Sjostrom H (2004) Deamidation and cross-linking of gliadin peptides by transglutaminases and the relation to celiac disease. Biochim Biophys Acta 1690:220-230.: 15511629