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Anti-D-dimer (human, dog) ABS 015-28

品牌：

貨號：ABS 015-28

規(guī)格：200 μg

貨期：

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Anti-D-dimer (human, dog)

商品詳情參考文獻相關資料

Cat. No.: ABS 015-28
Product name: Anti-D-dimer (human, dog)
Description: Mouse monoclonal antibody
Specificity: ABS 015-28 binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly coated fibrinogen.?
Immunogen: Purified human D-dimer
Application: ELISA, WB
Species reactivity (positive): Human, dog
Species reactivity (negative): Not determined
Subclass: IgG1/k
Gene ID: 2243, 475473
Clone number: 28
Epitope: When tested in a pair ?with itself, with ABS 015-22 and a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification: Protein-A purified
Form: Liquid
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8oC without exposure to light. No precautions necessary during handling.
Target: D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.

Application 1: ELISA
ABS 015-028 binds free D-dimer in solution.?

ABS 015-028 works as capture antibody in sandwich ELISA with biotinylated ABS 015-28 or ABS 015-22 as detection antibody.

Biotinylated ABS 015-028 works as detection antibody in sandwich ELISA with ABS 015-22 or ABS 015-28 as capture antibody.

The combination with ABS 015-28 as capture antibody and ABS 015-22B as detection antibody is recommended.
?

The calibration curve of a sandwich assay for D-Dimer using ABS 015-28 as capture antibody and ABS 015-22B as the biotinylated detection antibody. ?
Application 2: WB

Due to reducing conditions in Western blotting ABS 015-28 binds the beta-chain (40kDa) of D-Dimer, and not the entire D-Dimer complex.?