Concentration
3u/μl
5 ' . . . A G↓C T. . . 3 '
3 ' . . . T C↑G A . . . 5 '
Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.
Unit Definition
3u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37?C in 20μ l or 50μl of assay buffer.
Thermal Inactivation
65?C for 20 minutes.
Quality Control Assays
Ligation/ Recutting Assay
After 3-fold overdigestion with Alu I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 6u o f Alu I for 16 hours at 37?C.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
FDRE1120
|
Alu I
|
25 preps
|
Downloads
Manual
Alu I
