Concentration
10u/μl
5 ' . . . G↓G TA C C . . . 3 '
3 ' . . . C C AT G↑G . . . 5 '
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol
Unit Definition
10u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37?C in 20μl or 50μl of assay buffer.
Thermal Inactivation
65?C for 20 minutes.
Quality Control Assays
Ligation/ Recutting Assay
After 10-fold overdigestion with Acc65 I, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Acc65 I for 16 hours at 37?C.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
FDRE1104
|
Acc65 I (Kpn I*)
|
50 preps
|
Downloads
Manual
Acc65 I (Kpn I*)
