Description
The ViPrimePLUS Canine Herpesvirus qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Canine Herpes Virus genome in organ tissues such as liver, spleen, lungs and kidneys, faeces, blood, urine, vaginal and penile secretions, and nasal and oral discharges.
Canine Herpesvirus (CHV) is a double-stranded DNA virus that causes fatal haemorrhagic diseases in puppies that are less than 3 weeks old. The mortality rate is often reported as 100% in the affected litter. Survivors are usually left with cardiac and brain damage or other neurological disease. Infection in *****s is usually asymptomatic and mild resulting in sores in the genital area, ocular and respiratory discomforts. The virus can be transmitted vertically during birth giving from the infected dam to the neonates or horizontally during mating and oronasal contact. Lower temperature tends to stimulate aggressive viral replication. Therefore, newborns before weaning (less than 1 week old) are more susceptible to severe disease manifestation than *****s due to their lack of ability to regulate body temperature. Infected female dogs may develop antibodies and pass them on to the litters subsequent to the first infected litter. Puppies that receive maternal antibodies may be infected with the virus, but disease does not result. Vaccine available is not effective in providing lifelong protection and antiviral drugs have been generally unsuccessful.
ViPrimePLUS Canine Herpes Virus qPCR Kit is intended for research use only.
Kit Component
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CHV Primers and Probe Mix (CHV PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Canine samples (e.g. liver, spleen, lungs and kidneys, faeces, blood, urine, vaginal and penile secretions, and nasal, oral discharges)
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Gene Target
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Glycoprotein B gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QV2004
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ViPrimePLUS Canine Herpesvirus qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Canine Herpesvirus qPCR Kit
