Description
The ViPrimePLUS MRSA qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of MRSA genomes in clinical samples of the throat, nose groin, perineum and wound.
Staphylococcus aureus is a Gram positive, cocci bacterium that resides in the mucosal layer and moist skin area, particularly in nose, axilla and perineum. About 30% of the human population is colonized by the commensal pathogen without any symptoms. Due to the large reservoir and the ease of transmission via skin contact, S. aureus is considered a nuisance in hospital settings. This nosocomial infection causes postoperative wound infection, superficial abscesses, boils, Ritter disease, osteomyelitis, septicemia, toxic shock syndrome and pneumonia. MRSA is a group of S. aureus resistant to penicillin, methicillin and a narrow range of antibiotics due to the acquisition of mecA gene. MSSA (methicillin susceptible S. aureus) refers to the non-methicillin resistant strains. Stronger antibiotics are being sought after, as intermediate resistant strain against vancomycin was found. Besides, its interbreeding nature with some community staph produces the invasive and resistant community-acquired MRSA. Therefore, screening and control of antibiotic usage are essential to contain the infection outbreak.
ViPrimePLUS MRSA qPCR Kit is intended for research use only.
Kit Component
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MRSA Primers and Probe Mix (MRSA PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. throat, nose groin, perineum, wound)
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Gene Target
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mecA (penicillin binding protein 2) and S. aureus femB gene (chromosomal gene)
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2038
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ViPrimePLUS MRSA qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS MRSA qPCR Kit
