Description
The ViPrimePLUS Mycobacterium tuberculosis complex qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the Mycobacterium tuberculosis complex genomes in clinical samples (e.g. sputum, nasopharyngeal fluid and throat swab).
Tuberculosis (TB), a lower respiratory tract infection, is the second lead killer of infectious disease and 1.4million deaths were reported in 2011. About a third of the world's population suffered from latent TB, where there are 10% chances for them to fall ill to the disease. The M. tuberculosis complex consists of Mycobacterium tuberculosis (the main cause of TB), M. africanu, M. canetti, M. bovis and M. microti in which all causes TB in humans and some in animals such as M. bovis in bovine and M. microti in vole. Immunocompromised patients and smokers seem to have increased risk for developing active TB infection. The disease starts with mild symptoms thus making it difficult for early treatment. TB and HIV co-infection is a major concern as patient is 21 to 34 times more likely to develop active TB disease and almost 25% of deaths in AIDS patients are due to TB. The treatment is usually via a course of antibiotic administration. However, the evolution of multidrug resistant TB strains has required the use of stronger drugs for treatment.
ViPrimePLUS Mycobacterium tuberculosis complex qPCR Kit is intended for research use only.
Kit Component
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TB Primers and Probe Mix (TB PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. sputum, nasopharyngeal fluid, throat swab)
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Gene Target
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IS6110 repeat region
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2041
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ViPrimePLUS Mycobacterium tuberculosis complex qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Mycobacterium tuberculosis complex qPCR Kit
