Description
The ViPrimePLUS Vibrio qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of all Vibrio species genomes in food or water samples.
Vibrio (all species) is a Gram negative, comma-shaped bacterium that is commonly found in marine environment as most of the species are halophilic. There are at least 12 human pathogenic species naturally occur in tropical and the 3 commonly known foodborne pathogen are V. cholera, V. parahaemolyticus and V. vulnificus. Infections with the former 2 species will cause gastrointestinal disease while V. vulnificus can cause septicemia. V. cholerae can be introduced to the freshwater via fecal contamination. Therefore, routine screening is important to ensure safe water supply.
ViPrimePLUS Vibrio qPCR Kit is intended for research use only.
Kit Component
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Vibrio Primers and Probe Mix (Vibrio PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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? Food samples
? Water samples
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Gene Target
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RNA polymerase alpha subunit (rpoA) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2067
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ViPrimePLUS Vibrio qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Vibrio qPCR Kit
