Description
The ViPrimePLUS Streptococcus pneumoniae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Streptococcus pneumoniae genomes in clinical samples (e.g. throat swab, cerebrospinal fluids and blood).
Streptococcus pneumoniae is a Gram positive, alpha-hemolytic streptococcal bacterium. It has more than 91 serotypes, where only several cause disease in human. S. pneumoniae and S. viridans are distinguished with opthocin test where the former is sensitive to opthocin. Currently, S. pneumoniae is the leading cause of pneumonia, other than Haemophilus influenzae type b. About 1.2 million child deaths are recorded each year due to pneumonia. Young children, elderly, immunocompromised patients and people with chronic medical conditions, are more susceptible to the pneumococcal pneumonia. Besides that, S. pneumoniae is also the most common cause of bacterial meningitis in *****s and children. Mode of transmission is via contact with respiratory droplets. Vaccines have been developed to prevent the pneumococcal disease.
ViPrimePLUS Streptococcus pneumoniae qPCR Kit is intended for research use only.
Kit Component
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S. pneumoniae Primers and Probe Mix (S. pneumoniae PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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DNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. throat swab, cerebrospinal fluids and blood)
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Gene Target
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Glucosyltransferase-1 (gtfR) gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM2062
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ViPrimePLUS Streptococcus pneumoniae qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Streptococcus pneumoniae qPCR Kit
