Description
The ViPrimePLUS Shewanella putrefaciens qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Shewanella putrefaciens genomes in clinical (e.g. blood), seafood and water samples.
Shewanella putrefaciens is a Gram negative bacterium which causes opportunistic infection, particularly in immunocompromised patients. Its natural habitat is in the marine environment, favoring warm climate. Infection with the pathogen usually causes skin ulceration and soft tissue infection. In more severe cases, hepatobiliary disease, fatal peritonitis, necrotizing fasciitis and bacteremia may occur, causing devastating damage. Most of the Shewanella infections can be treated by a combination of surgical therapy, drainage and antibiotics.
ViPrimePLUS Shewanella putrefaciens qPCR Kit is intended for research use only.
Kit Component
-
S. putrefaciens Primers and Probe Mix (S. putrefaciens PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
DNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
? Clinical samples (e.g. blood)
? Seafood samples
? Water samples
|
|
Gene Target
|
ferF pseudogene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM2052
|
ViPrimePLUS Shewanella putrefaciens qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Shewanella putrefaciens qPCR Kit
