Description
The ViPrimePLUS Hepatitis Delta Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Hepatitis Delta Virus genome in clinical samples (e.g. blood plasma and serum).
Hepatitis D virus is a circular, single stranded RNA virus which requires hepadnavirus, i.e. HBV to replicate and cause infection as it does not cause disease by itself. Thus, HBV carriers are most susceptible to HDV infection. Co-infection or superinfection by HDV results in both acute and chronic disease. In rare cases, fulminant acute infection may lead to hepatitis encephalopathy. Chronic infection may cause hepacellular carcinoma, where liver transplant is the only cure due to the absence of anti-viral drug. Transmission of the virus is via blood. Routine screening could prevent irreversible liver damage via early illness management.
ViPrimePLUS Hepatitis Delta Virus RT-qPCR Kit is intended for research use only.
Kit Component
-
HDV Primers and Probe Mix (HDV PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
RNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
Clinical samples (e.g. blood plasma, serum)
|
|
Gene Target
|
Large HD antigen encoding gene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM5013
|
ViPrimePLUS Hepatitis Delta Virus RT-qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Hepatitis Delta Virus RT-qPCR Kit
