Description
The ViPrimePLUS Polyomavirus BK qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Polyomavirus BK genomes in clinical samples (e.g. blood, tissues, urine and respiratory fluids).
Polyomaviruses are double-stranded DNA virus that is potentially oncogenic as implicated in the name poly (multiple) -oma (tumour). There are 3 polyomaviruses most relevant to human disease, i.e. JC virus, BK virus and Simian Virus 40 (SV40). While SV40 is a common pathogen to monkeys, JC and BK virus are ubiquitous in human where primary infections typically occur in childhood (3-4years old for BK virus and 7-10years old for JC virus). Signs of infection are mild and asymptomatic. After the initial infection, the viruses established latency in various tissues, especially the renal tissue. Reactivation may occur in immunocompromised individuals resulting in respiratory and urinary tract disease by BK virus and meningoencephalitis by JC virus infection. The potentially tumorigenic SV40 virus was introduced to human population via contaminated polio vaccines produced from SV40-infected monkey cells. Detection of those viruses in the kidney, bone tissues and peripheral blood suggested organ transplantation and blood transfusion as the routes for horizontal transmission in human, aside from the fecal-oral route.
ViPrimePLUS Polyomavirus BK qPCR Kit is intended for research use only.
Kit Component
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BK Primers and Probe Mix (BK PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. blood, tissues, urine, respiratory fluids)
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Gene Target
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Non-coding region of the BK virus genome
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5065
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ViPrimePLUS Polyomavirus BK qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Polyomavirus BK qPCR Kit
