Description
The ViPrimePLUS Norovirus genotype 1 RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Norovirus genotype 1 genome in clinical (e.g. stool), food samples and environmental water samples.
Norovirus is a small, single-stranded RNA virus, which is also known as the Norwalk virus that is responsible for the 80% non-bacterial outbreaks of acute gastroenteritis worldwide and prone to occur during winter season. The symptoms of the disease include vomiting, diarrhea, abdominal cramps and fever that normally lasted for 2 days. It affects individuals of all ages, however prolong infection may occur in children and elderly. The virus is shed in stool by infected patients, thus is transmitted through contacting with contaminated surfaces and ingesting contaminated water and food. The ease of transmission and low infection dose bring to major outbreaks in many environmental settings. There are 5 recognized genogroups, in which GI, GII and GIV affect human. Under GI, there are 7 strains and GII consists of 12 strains. The recently emerge GII.4 Sydney strain is recognized as the main cause of norovirus outbreak in the U.S.
ViPrimePLUS Norovirus genotype 1 RT-qPCR Kit is intended for research use only.
Kit Component
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NoV G1 Primers and Probe Mix (NoV G1 PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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? Clinical samples (e.g. stools)
? Food samples
? Environmental samples
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Gene Target
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Capsid protein gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5062
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ViPrimePLUS Norovirus genotype 1 RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Norovirus genotype 1 RT-qPCR Kit
