Description
The ViPrimePLUS Mumps Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Mumps Virus genome in clinical samples (e.g. throat swab, cerebrospinal fluid and urine).
Mumps virus is a linear, non-segmented, negative-strand RNA virus that belongs to the genus Rubulavirus in the family Paramyxoviridae The mumps virus genome contains seven genes encoding the nucleoprotein (N), phosphoprotein (P), matrix protein (M), fusion protein (F), small hydrophobic protein (SH), hemagglutinin-neuraminidase (HN) and polymerase protein (L). It is transmitted either by direct contact or aerosol route, but could also spread by contact with contaminated objects. The virus enters the body via the pharynx or the conjunctiva and upon receptor binding to sialic acid located on the host cells, the nucleocapsid is released into the host cells through membrane fusion. The virus then localizes in the throat but disseminates via the bloodstream, infecting various organs, salivary glands, testes, ovaries, pancreas and the brain. The period of incubation for mumps virus ranges from 12 to 24 days, with infection being asymptomatic in 20-30% cases. These symptoms include fever, headache, malaise, face pain, unilateral or bilateral swelling of the parotid glands and jaws, and orchitis in males.
ViPrimePLUS Mumps Virus RT-qPCR Kit is intended for research use only.
Kit Component
-
Mumps Primers and Probe Mix (Mumps PPM)
-
Positive Control
-
Internal Extraction Control Primers and Probe Mix (IEC PPM)
-
Internal Extraction Control (IEC DNA)
-
Nuclease Free Water
-
Template Preparation Buffer
Product Specification
|
Technology
|
Taqman probe-based real-time PCR assay
|
|
Type of Nucleic Acid Kit
|
RNA
|
|
Kit Storage
|
Shipped in room temperature, store at -20℃ on arrival
|
|
Sample Material
|
Clinical samples (e.g. throat swab, cerebrospinal fluid, urine)
|
|
Gene Target
|
Small hydrophobic protein (SH) gene
|
|
Detection Limit
|
10 to <100 copies per reaction
|
|
Sensitivity & Specificity
|
Ct value 28±3 with probability of 95%
|
|
Controls Included
|
? Internal Extraction Control (IEC) as inhibition control
? Positive control
|
|
Channels
|
? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
|
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
QM5061
|
ViPrimePLUS Mumps Virus RT-qPCR Kit
|
150 reactions
|
Download
Manual
ViPrimePLUS Mumps Virus RT-qPCR Kit
