Description
The ViPrimePLUS Human Coronavirus Group 1b RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Coronavirus Group 1b genome in clinical samples (e.g. throat swab, nasopharyngeal aspirates and stool).
Coronavirus family represents a large collection of viruses. These single-stranded RNA viruses are divided into groups and subgroups based on their genomic make-up. The human health-concerned coronaviruses include the group 1b Human Coronavirus NL63 and 229E, group 2a Human Coronavirus OC43 and Coronavirus HKU1 and group 2b SARS virus. The infections will result in respiratory illnesses such as common cold, SARS (Severe Acute Respiratory Syndrome) and gastrointestinal diseases. The rest of the coronaviruses are isolated from bat, bovine, porcine, feline or avian. The emergence of a novel coronavirus in the Middle East region recently has gained the world's attention due to its epidemic potential, for example SARS outbreaks in 2002.
ViPrimePLUS Human Coronavirus Group 1b RT-qPCR Kit is intended for research use only.
Kit Component
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HCoV_Gp1 Primers and Probe Mix (HCoV_Gp1 PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. throat swab, nasopharyngeal aspirates, stool)
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Gene Target
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Replicase polyprotein 1ab gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5016
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ViPrimePLUS Human Coronavirus Group 1b RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Human Coronavirus Group 1b RT-qPCR Kit
