Description
The ViPrimePLUS Human Rhinovirus 1B RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Human Rhinovirus 16 genome in clinical samples (e.g. sputum, nasopharyngeal aspirate and throat swab).
Human Rhinovirus (HRV) is a single-stranded RNA virus which causes 50% of the common cold cases in human. There are 3 genetically distinct groups of HRV, i.e. group A, B and C, where approximately 100 serotypes are described. HRV 1B belongs to group A. Wheezing is the frequent manifestation of lower respiratory tract infection by HRV in young children. It is also associated with upper respiratory tract infection, otitis media, and sinusitis. Recent evidence suggests that infection with HRV-C may result in more severe disease. HRV-C has a global distribution, with a prevalence intermediate with HRV-A and HRV-B. There are currently no approved antiviral therapies for HRVs, and treatment remains primarily supportive.
ViPrimePLUS Human Rhinovirus 1B RT-qPCR Kit is intended for research use only.
Kit Component
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HRV1B Primers and Probe Mix (HRV1B PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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Clinical samples (e.g. sputum, nasopharyngeal aspirate, throat swab)
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Gene Target
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Polyprotein gene in the RNA genome
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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? Internal Extraction Control (IEC) as inhibition control
? Positive control
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Channels
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? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5051
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ViPrimePLUS Human Rhinovirus 1B RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Human Rhinovirus 1B RT-qPCR Kit
