最近最新的日本中文MV_麻豆出品视频在线_超pen个人视频97_国产精品第一国产精品_国产香蕉片_免费看麻豆文化传媒_奶大灬舒服灬太大了一进一出_人妻激情偷乱一区二区三区

Concentration
10-30u/μl 
5'…G↓GTACC…3' 
3'…CCATG↑G…5'

Reaction Conditions 
1X Buffer V4 
10mM Tris-HCI (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCI, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with Acc65I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Acc65I for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent) 
*Blocked by overlapping dcmˉ methylation (CmCWGG): GGTACCWGG

Ordering Information

Downloads
Manual
Acc65I {KpnI*}