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Concentration
1-10u/μl 
5'…TGC↓GCA…3' 
3'…ACG↑CGT…5'

Reaction Conditions 
1X Buffer UB 
25mM Tris-acetate (pH 7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7mM 2-mercaptoethanol, and 50μg /ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA, and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with Acc16l, more than 80% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Acc16I for 16 hours at 37°C
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
Acc16I {MstI}