Concentration
5u/μl
5'…CC↓TNAGC…3'
3'…GGANT↑CG…5'
Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20°C.
Thermal Inactivation
80°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with Bpu10I, 80% of the DNA fragments can be ligated in the presence of 10% PEG and of these 90% can be recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Bpu10I for 16 hours at 37°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
*High enzyme concentration may result in Star Activity.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1158
|
Bpu10I
|
100u
|
Downloads
Manual
Bpu10I
