Concentration
1-5u/μl
5'…C↓GGCCG…3'
3'…GCCGG↑C…5'
Reaction Conditions
1X Buffer V3
50mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 100mM NaCl, and 100μg/ml BSA. Incubate at 50°C.
Storage Buffer
10mM Tris-HCl (pH 8.2), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C. Store at -70°C for period longer than 90 days.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 5-fold overdigestion with BseX3I, 90% of the DNA fragments can be ligated and of these 80% can be recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BseX3I for 16 hours at 50°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
* Enzyme is stable for up to 6 months if properly stored. It is recommended that the enzyme is kept in small aliquots to avoid repeated freeze-thawed cycles.
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1178
|
BseX3I
|
100u
|
Downloads
Manual
BseX3I
