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Concentration
1-10u/μl 
5'…GCAATGNN↓…3' 
3'…CGTTAC↑NN…5

Reaction Conditions 
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg/ml BSA. Incubate at 60°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 10-fold overdigestion with Bse3D I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Bse3D I 16 hours at 60°C. 
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bse3D I {BsrDI}