Concentration
1-4u/μl
5'…G↓CGCGC…3'
3'…CGCGC↑G…5'
Reaction Conditions
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl2, 50mM NaCl, and 100μg/ml BSA. Incubate at 50°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 4-fold overdigestion with BsePI, 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 8u of BsePI for 16 hours at 50°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1176
|
BsePI
|
100u
|
Downloads
Manual
BsePI
