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Concentration
10- 30u/μl
5'…C↓CATGG…3' 
3'…GGTAC↑C…5'

Reaction Conditions 
1X Buffer Bsp19I
20mM Tris-HCl (pH8.5), 10mM MgCl₂, 200mM NaCl and 1mM DTT. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes

Ligation / Recutting Assay 
After 10-fold overdigestion with Bsp19I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Bsp19I for for 16 hours at 37°C. 
Supplied with 10X Buffer Bsp19I, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bsp19I {NcoI}