Concentration
2-5u/μl
5'…CCTNN↓NNNAGG…3'
3'…GGANNN↑NNTCC…5'
Reaction Conditions
1X Buffer UB
25mM Tris-acetate (pH 7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7mM 2-mercaptoethanol, and 50μg/ml BSA. Incubate at 65°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
80°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with BstENI, 60% of the DNA fragments can be ligated and of these 90% can be recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 3u of BstENI for 16 hours at 65°C.
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
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Catalog No
|
Description
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Pack Size
|
|
RE1216
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BstENI {EcoNI}
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100u
|
Downloads
Manual
BstENI {EcoNI}
Publication
This Product Has Been Used In:
Karimi, M.H., Salek, S., Yaghobi, R., Ramzi, M., Geramizadeh, B., Hejr, S. (2014). Association of IL-17 gene polymorphisms and serum level with graft versus host disease after allogeneic hematopoietic stem cell transplantation. Cytokine.69. Pp..120-124
