Concentration
50u/μl
5'…GCG↓C…3'
3'…C↑GCG…5'
Reaction Conditions
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 50°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
None.
Ligation / Recutting Assay
After 50-fold overdigestion with BstHHI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 100u of BstHHI for 16 hours at 50°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by CpG methylation
Ordering Information
|
Catalog No
|
Description
|
Pack Size
|
|
RE1224
|
BstHHI {HhaI}
|
1000u
|
Downloads
Manual
BstHHI {HhaI}
Publication
This Product Has Been Used In:
Badouei, M.A., Jajarmi, M., Mirsalehian, A., (2015) Virulence profiling and genetic relatedness of Shigatoxin-producing Escherichia coli isolated from humans and ruminants. Comparative Immunology, Microbiology and Infectious Diseases. 38. Pp15-20.
Chemkhi, J., Souguir, H., Ali, I.B.H., Driss, M., Guizani, I., Gouerbouj, S. (2015) Natural infection of Algerian hedgehog, Atelerix algirus (Lereboullet1842) with Leishmania parasites in Tunisia. Acta Tropica. 150. Pp42-51.
