Concentration
30-100u/μl
5'…GTCTC(N)1↓…3'
3'…CAGAG(N)5↑…5'
Reaction Conditions
1X Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 55°C.
Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
Thermal Inactivation
65°C for 20 minutes.
Ligation / Recutting Assay
After 30-fold overdigestion with BstMAI, more than 90% of the DNA fragments can be ligated and recut.
Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 30u of BstMAI for 16 hours at 55°C.
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
Ordering Information
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Catalog No
|
Description
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Pack Size
|
|
RE1226
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BstMAI {BsmAI}
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1000u
|
Downloads
Manual
BstMAI {BsmAI}
Publication
This Product Has Been Used In:
Ruzilawati, A.B., Mohm Suhaimi, A.W., Gan, S.H. (2007) . Genetic polymorphisms of CYP3A4: CYP3A4?18 allele is found in five healthy Malaysian subjects. Clinica Chimica Acta. 383(1-2) pp.158-162
