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Concentration
20-50u/μl
5'…GACNN↓NNGTC…3'
3'…CTGNN↑NNCAG…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 65°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 5-fold overdigestion with BstPA I, less than 5% of the DNA fragments can be ligated.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of BstPAI for 16 hours at 25°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent) 
*Prolong Incubation will result in Star Activity.

Ordering Information

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Manual
BstPAI {PshAI}